| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human NFRKB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
NFRKB |
| Gene ID: |
4798 |
| Uniprot ID: |
NFRKB_HUMAN |
| Specificity: |
This kit recognizes Human NFR Kappa B in samples. No significant cross-reactivity or interference between Human NFR Kappa B and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in thymus, brain, testes, spleen and liver. |
| Function | Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'. Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Modulates the deubiquitinase activity of UCHL5 in the INO80 complex. |
| Protein Name | Nuclear Factor Related To Kappa-B-Binding ProteinDna-Binding Protein R Kappa-BIno80 Complex Subunit G |
| Database Links | Reactome: R-HSA-5689603Reactome: R-HSA-5696394 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Nuclear Factor Related To Kappa-B-Binding Protein CLIA kitDna-Binding Protein R Kappa-B CLIA kitIno80 Complex Subunit G CLIA kitNFRKB CLIA kitINO80G CLIA kit |
| Specificity | This kit recognizes Human NFRκB in samples. No significant cross-reactivity or interference between Human NFRκB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-109 | 100 |
| EDTA plasma(n=8) | 95-111 | 101 |
| Cell culture media(n=8) | 95-109 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.13 | 3.62 | 19.58 | 2.12 | 3.87 | 21.40 |
| Standard deviation | 0.20 | 0.27 | 1.61 | 0.23 | 0.42 | 2.24 |
| CV (%) | 9.39 | 7.46 | 8.22 | 10.85 | 10.85 | 10.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
