| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This NES1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
NUCB2 |
| Gene ID: |
4925 |
| Uniprot ID: |
NUCB2_HUMAN |
| Specificity: |
This kit recognizes Human NES1 in samples. No significant cross-reactivity or interference between Human NES1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Calcium-binding protein which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3. Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. |
| Protein Name | Nucleobindin-2Dna-Binding Protein NefaEpididymis Secretory Protein Li 109Gastric Cancer Antigen Zg4Prepronesfatin Cleaved Into - Nesfatin-1 |
| Cellular Localisation | Golgi ApparatusMembranePeripheral Membrane ProteinCytoplasmSecretedEndoplasmic ReticulumNucleus EnvelopeGolgi Retention Is Mediated By Its N-Terminal RegionNesfatin-1: Secreted |
| Alternative ELISA Names | Nucleobindin-2 ELISA kitDna-Binding Protein Nefa ELISA kitEpididymis Secretory Protein Li 109 ELISA kitGastric Cancer Antigen Zg4 ELISA kitPrepronesfatin Cleaved Into - Nesfatin-1 ELISA kitNUCB2 ELISA kitNEFA ELISA kit |
| Specificity | This kit recognizes Human NES1 in samples. No significant cross-reactivity or interference between Human NES1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 94-109 | 99 |
| Cell culture media (n=8) | 91-107 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.80 | 141.45 | 380.38 | 49.78 | 145.73 | 387.06 |
| Standard deviation | 2.87 | 6.86 | 19.86 | 3.35 | 8.39 | 20.20 |
| CV (%) | 5.54 | 4.85 | 5.22 | 6.73 | 5.76 | 5.22 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
