| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.062ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This NEIL1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
NEIL1 |
| Gene ID: |
79661 |
| Uniprot ID: |
NEIL1_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | |
| Function | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC. |
| Protein Name | Endonuclease 8-Like 1Dna Glycosylase/Ap Lyase Neil1Dna-(Apurinic Or Apyrimidinic Site Lyase Neil1Endonuclease Viii-Like 1Fpg1Nei Homolog 1Neh1Nei-Like Protein 1 |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-5649702Reactome: R-HSA-9616334 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleusChromosomeDuring MitosisAssociates With Centrosomes And Condensed Chromatin |
| Alternative ELISA Names | Endonuclease 8-Like 1 ELISA kitDna Glycosylase/Ap Lyase Neil1 ELISA kitDna-(Apurinic Or Apyrimidinic Site Lyase Neil1 ELISA kitEndonuclease Viii-Like 1 ELISA kitFpg1 ELISA kitNei Homolog 1 ELISA kitNeh1 ELISA kitNei-Like Protein 1 ELISA kitNEIL1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
