| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.122ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This NAAA Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
NAAA |
| Gene ID: |
27163 |
| Uniprot ID: |
NAAA_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas. |
| Post Translational Modifications | N-glycosylated. Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine. A disulfide bond is seen in the crystal structure of the human protein, but the Cys residues are not conserved in rodents. Autoproteolytic cleavage at pH 4.5 gives rise to the alpha and beta subunit. Cleavage gives rise to a conformation change that activates the enzyme. The same catalytic Cys residue mediates the autoproteolytic cleavage and subsequent hydrolysis of lipid substrates (Probable). |
| Function | Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine. |
| Protein Name | N-Acylethanolamine-Hydrolyzing Acid AmidaseAcid Ceramidase-Like ProteinAcylsphingosine Deacylase NaaaN-Acylsphingosine Amidohydrolase-LikeAsah-Like Protein Cleaved Into - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Alpha - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Beta |
| Database Links | Reactome: R-HSA-112310 |
| Cellular Localisation | LysosomeMembranePeripheral Membrane Protein |
| Alternative ELISA Names | N-Acylethanolamine-Hydrolyzing Acid Amidase ELISA kitAcid Ceramidase-Like Protein ELISA kitAcylsphingosine Deacylase Naaa ELISA kitN-Acylsphingosine Amidohydrolase-Like ELISA kitAsah-Like Protein Cleaved Into - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Alpha - N-Acylethanolamine-Hydrolyzing Acid Amidase Subunit Beta ELISA kitNAAA ELISA kitASAHL ELISA kitPLT ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
