• Human MYOC (Myocilin) Sandwich ELISA Kit (STJE0010849)

Human MYOC (Myocilin) Sandwich ELISA Kit (STJE0010849)

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STJE0010849

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.28ng/mL
Detection Limit: 0.781-50ng/mL
Short Description: This MYOC Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: MYOC
Gene ID: 4653
Uniprot ID: MYOC_HUMAN
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Detected in aqueous humor. Detected in the eye (at protein level). Widely expressed. Highly expressed in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, and bone marrow-derived mesenchymal stem cells. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and seems to be expressed at higher levels than in normal eyes, regardless of the type or clinical severity of glaucoma. The myocilin 35 kDa fragment is detected in aqueous humor and to a lesser extent in iris and ciliary body.
Post Translational Modifications Different isoforms may arise by post-translational modifications. Glycosylated. Palmitoylated. Undergoes a calcium-dependent proteolytic cleavage at Arg-226 by CAPN2 in the endoplasmic reticulum. The result is the production of two fragments, one of 35 kDa containing the C-terminal olfactomedin-like domain, and another of 20 kDa containing the N-terminal leucine zipper-like domain.
Function Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.
Protein Name Myocilin
Myocilin 55 Kda Subunit
Trabecular Meshwork-Induced Glucocorticoid Response Protein Cleaved Into - Myocilin - N-Terminal Fragment
Myocilin 20 Kda N-Terminal Fragment - Myocilin - C-Terminal Fragment
Myocilin 35 Kda N-Terminal Fragment
Database Links
Cellular Localisation Secreted
Golgi Apparatus
Cytoplasmic Vesicle
Extracellular Space
Extracellular Matrix
Extracellular Exosome
Mitochondrion
Mitochondrion Intermembrane Space
Mitochondrion Inner Membrane
Mitochondrion Outer Membrane
Rough Endoplasmic Reticulum
Cell Projection
Cilium
Located Preferentially In The Ciliary Rootlet And Basal Body Of The Connecting Cilium Of Photoreceptor Cells
And In The Rough Endoplasmic Reticulum
It Is Only Imported To Mitochondria In The Trabecular Meshwork
Localizes To The Golgi Apparatus In Schlemm's Canal Endothelial Cells
Appears In The Extracellular Space Of Trabecular Meshwork Cells By An Unconventional Mechanism
Likely Associated With Exosome-Like Vesicles
Localizes In Trabecular Meshwork Extracellular Matrix
Myocilin
C-Terminal Fragment: Secreted
N-Terminal Fragment: Endoplasmic Reticulum
Remains Retained In The Endoplasmic Reticulum
Alternative ELISA Names Myocilin ELISA kit
Myocilin 55 Kda Subunit ELISA kit
Trabecular Meshwork-Induced Glucocorticoid Response Protein Cleaved Into - Myocilin - N-Terminal Fragment ELISA kit
Myocilin 20 Kda N-Terminal Fragment - Myocilin - C-Terminal Fragment ELISA kit
Myocilin 35 Kda N-Terminal Fragment ELISA kit
MYOC ELISA kit
GLC1A ELISA kit
TIGR ELISA kit
output

Information sourced from Uniprot.org

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