| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human MYH10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MYH10 |
| Gene ID: |
4628 |
| Uniprot ID: |
MYH10_HUMAN |
| Specificity: |
This kit recognizes Human MYH10 in samples. No significant cross-reactivity or interference between Human MYH10 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. |
| Post Translational Modifications | Phosphorylated by ABL2. |
| Function | Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension.this function is mechanically antagonized by MYH9. |
| Protein Name | Myosin-10Cellular Myosin Heavy Chain - Type BMyosin Heavy Chain 10Myosin Heavy Chain - Non-Muscle IibNon-Muscle Myosin Heavy Chain BNmmhc-BNon-Muscle Myosin Heavy Chain IibNmmhc Ii-BNmmhc-Iib |
| Database Links | Reactome: R-HSA-3928663Reactome: R-HSA-416572Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5627123 |
| Cellular Localisation | Cell ProjectionLamellipodiumColocalizes With Mcc At The Leading Edge Of Migrating Cells |
| Alternative CLIA Names | Myosin-10 CLIA kitCellular Myosin Heavy Chain - Type B CLIA kitMyosin Heavy Chain 10 CLIA kitMyosin Heavy Chain - Non-Muscle Iib CLIA kitNon-Muscle Myosin Heavy Chain B CLIA kitNmmhc-B CLIA kitNon-Muscle Myosin Heavy Chain Iib CLIA kitNmmhc Ii-B CLIA kitNmmhc-Iib CLIA kitMYH10 CLIA kit |
| Specificity | This kit recognizes Human MYH10 in samples. No significant cross-reactivity or interference between Human MYH10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-100 | 91 |
| EDTA plasma(n=8) | 96-111 | 103 |
| Cell culture media(n=8) | 94-109 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 94.83 | 216.35 | 805.12 | 93.87 | 224.66 | 791.66 |
| Standard deviation | 9.67 | 17.09 | 85.58 | 9.57 | 19.68 | 52.49 |
| CV (%) | 10.20 | 7.90 | 10.63 | 10.19 | 8.76 | 6.63 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
