| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human MUCL1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MUCL1 |
| Gene ID: |
118430 |
| Uniprot ID: |
MUCL1_HUMAN |
| Specificity: |
This kit recognizes Human SBEM in samples. No significant cross-reactivity or interference between Human SBEM and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in mammary, salivary glands and prostate. Also detected in lung. Mainly expressed in cancer cell lines of breast origin. Highly expressed in lymph node-positive compared with node-negative tumors. Detected in all lymph node containing metastatic cells. |
| Post Translational Modifications | O-glycosylated. |
| Function | May play a role as marker for the diagnosis of metastatic breast cancer. |
| Protein Name | Mucin-Like Protein 1Protein Bs106Small Breast Epithelial Mucin |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-977068 |
| Cellular Localisation | SecretedMembrane |
| Alternative CLIA Names | Mucin-Like Protein 1 CLIA kitProtein Bs106 CLIA kitSmall Breast Epithelial Mucin CLIA kitMUCL1 CLIA kitSBEM CLIA kitUNQ590 CLIA kitPRO1160 CLIA kit |
| Specificity | This kit recognizes Human SBEM in samples. No significant cross-reactivity or interference between Human SBEM and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 93 |
| EDTA plasma(n=8) | 92-108 | 99 |
| Cell culture media(n=8) | 99-113 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 204.27 | 549.60 | 1864.38 | 222.09 | 564.48 | 1935.30 |
| Standard deviation | 22.20 | 46.39 | 191.66 | 24.92 | 65.54 | 217.91 |
| CV (%) | 10.87 | 8.44 | 10.28 | 11.22 | 11.61 | 11.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
