| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human MUC17 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MUC17 |
| Gene ID: |
140453 |
| Uniprot ID: |
MUC17_HUMAN |
| Specificity: |
This kit recognizes Human MUC17 in samples. No significant cross-reactivity or interference between Human MUC17 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed almost exclusively in the intestine. Expression is especially high in both the duodenum and transverse colon. Expressed in mature absorptive cells of the small intestinal villi. No expression is detected in goblet cells. Highly expressed in pancreatic adenocarcinoma tissue (at protein level). Expression is not detectable in normal pancreas, in pancreatitis or in cell lines derived from other cancers. |
| Post Translational Modifications | Probably cleaved within the SEA domain. N-glycosylated. Contains high mannose and complex-type glycans. The forms containing the complex type glycans localize to the cell surface. Not O-glycosylated. |
| Function | Probably plays a role in maintaining homeostasis on mucosal surfaces. |
| Protein Name | Mucin-17Muc-17Small Intestinal Mucin-3Muc-3 |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-977068 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Membrane ProteinIsoform 2: SecretedCell Membrane |
| Alternative CLIA Names | Mucin-17 CLIA kitMuc-17 CLIA kitSmall Intestinal Mucin-3 CLIA kitMuc-3 CLIA kitMUC17 CLIA kitMUC3 CLIA kit |
| Specificity | This kit recognizes Human MUC17 in samples. No significant cross-reactivity or interference between Human MUC17 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-113 | 103 |
| EDTA plasma(n=8) | 87-100 | 93 |
| Cell culture media(n=8) | 90-104 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 41.25 | 127.99 | 322.69 | 39.11 | 122.09 | 354.35 |
| Standard deviation | 4.10 | 9.07 | 28.11 | 4.02 | 11.01 | 26.90 |
| CV (%) | 9.94 | 7.09 | 8.71 | 10.28 | 9.02 | 7.59 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
