| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human MUC13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MUC13 |
| Gene ID: |
56667 |
| Uniprot ID: |
MUC13_HUMAN |
| Specificity: |
This kit recognizes Human MUC13 in samples. No significant cross-reactivity or interference between Human MUC13 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. |
| Post Translational Modifications | Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homooligomerization into dimers is dependent on intrachain disulfide bonds. Highly N-glycosylated. |
| Function | Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. |
| Protein Name | Mucin-13Muc-13Down-Regulated In Colon Cancer 1 |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-9696264Reactome: R-HSA-9696270Reactome: R-HSA-9696273Reactome: R-HSA-977068 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinApical Cell MembraneSecretedAlso Exists As A Soluble Form |
| Alternative CLIA Names | Mucin-13 CLIA kitMuc-13 CLIA kitDown-Regulated In Colon Cancer 1 CLIA kitMUC13 CLIA kitDRCC1 CLIA kitRECC CLIA kitUNQ6194 CLIA kitPRO20221 CLIA kit |
| Specificity | This kit recognizes Human MUC13 in samples. No significant cross-reactivity or interference between Human MUC13 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 103 |
| EDTA plasma(n=8) | 87-97 | 92 |
| Cell culture media(n=8) | 101-118 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.49 | 318.77 | 718.85 | 102.12 | 324.40 | 766.54 |
| Standard deviation | 10.31 | 28.78 | 56.07 | 8.48 | 24.30 | 87.54 |
| CV (%) | 9.77 | 9.03 | 7.80 | 8.30 | 7.49 | 11.42 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
