| Post Translational Modifications | Proteolytically processed in two steps to generate the Macrophage-expressed gene 1 protein, processed form: cleaved by trypsin in proximity of the helical transmembrane domain releases the ectodomain into the lysosomal lumen to orient the pore-forming domain toward the endogenous membranes, and processed by the asparagine endopeptidase (LGMN). Monoubiquitinated in response to bacterial infection.ubiquitination is required for vesicular localization and antibacterial activity and can be blocked by bacterial cell cycle inhibiting factor (cif). |
| Function | Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection.shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide. Macrophage-expressed gene 1 protein, processed form: Pore-forming protein that plays a central role in antigen cross-presentation in dendritic cells by mediating delivery of antigens for cross-presentation. Dendritic cells bridge innate and adaptive immunity by capturing exogenous antigens on MHC class-I molecules and presenting them to naive CD8(+) T-cells. Acts by forming a pore in antigen-containing compartments, promoting the release of antigens into the cytosol, enabling generation of MHCI:peptide complexes and T-cell priming. |
| Protein Name | Macrophage-Expressed Gene 1 ProteinMacrophage Gene 1 ProteinMpg-1Perforin-2P-2 Cleaved Into - Macrophage-Expressed Gene 1 Protein - Processed Form |
| Cellular Localisation | Isoform 1: Cytoplasmic Vesicle MembraneMulti-Pass Membrane ProteinBacterial Infection Induces Translocation Of The Cytoplasmic Vesicles To Bacterium-Containing Phagocytic Vesicles And Fusing Of The VesiclesMacrophage-Expressed Gene 1 ProteinProcessed Form: Cytoplasmic VesiclePhagosome MembraneProteolytically Processed In LysosomesLeading To Its Maturation And Forms Pores In The Membrane Of Antigen-Containing PhagosomesIsoform 2: Secreted |
| Alternative Protein Names | Macrophage-Expressed Gene 1 Protein proteinMacrophage Gene 1 Protein proteinMpg-1 proteinPerforin-2 proteinP-2 Cleaved Into - Macrophage-Expressed Gene 1 Protein - Processed Form proteinMPEG1 protein |
Information sourced from Uniprot.org
