| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This MMRN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
MMRN1 |
| Gene ID: |
22915 |
| Uniprot ID: |
MMRN1_HUMAN |
| Specificity: |
This kit recognizes Human MMRN1 in samples. No significant cross-reactivity or interference between Human MMRN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The N-terminus is blocked. Extensively N-glycosylated. |
| Function | Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein. |
| Protein Name | Multimerin-1Emilin-4Elastin Microfibril Interface Located Protein 4Elastin Microfibril Interfacer 4Endothelial Cell Multimerin Cleaved Into - Platelet Glycoprotein Ia* - 155 Kda Platelet MultimerinP-155P155 |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Multimerin-1 ELISA kitEmilin-4 ELISA kitElastin Microfibril Interface Located Protein 4 ELISA kitElastin Microfibril Interfacer 4 ELISA kitEndothelial Cell Multimerin Cleaved Into - Platelet Glycoprotein Ia* - 155 Kda Platelet Multimerin ELISA kitP-155 ELISA kitP155 ELISA kitMMRN1 ELISA kitECM ELISA kitEMILIN4 ELISA kitGPIA* ELISA kitMMRN ELISA kit |
| Specificity | This kit recognizes Human MMRN1 in samples. No significant cross-reactivity or interference between Human MMRN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-96 | 91 |
| EDTA plasma (n=8) | 94-109 | 100 |
| Cell culture media (n=8) | 89-101 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 236.70 | 545.51 | 1682.79 | 222.73 | 492.13 | 1789.76 |
| Standard deviation | 16.31 | 31.31 | 83.30 | 11.96 | 22.83 | 69.98 |
| CV (%) | 6.89 | 5.74 | 4.95 | 5.37 | 4.64 | 3.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
