| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human MMRN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MMRN1 |
| Gene ID: |
22915 |
| Uniprot ID: |
MMRN1_HUMAN |
| Specificity: |
This kit recognizes Human MMRN1 in samples. No significant cross-reactivity or interference between Human MMRN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Synthesized by endothelial cells and megakaryocytes. Stored in platelet alpha granules and endothelial cell Weibel-Palade bodies, following activation of these cells, it is released and attached to megakaryocytes, platelets, endothelium and subendothelium of blood vessels. Not found in plasma. Found in vascular tissues such as placenta, lung, and liver. |
| Post Translational Modifications | The N-terminus is blocked. Extensively N-glycosylated. |
| Function | Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein. |
| Protein Name | Multimerin-1Emilin-4Elastin Microfibril Interface Located Protein 4Elastin Microfibril Interfacer 4Endothelial Cell Multimerin Cleaved Into - Platelet Glycoprotein Ia* - 155 Kda Platelet MultimerinP-155P155 |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Multimerin-1 CLIA kitEmilin-4 CLIA kitElastin Microfibril Interface Located Protein 4 CLIA kitElastin Microfibril Interfacer 4 CLIA kitEndothelial Cell Multimerin Cleaved Into - Platelet Glycoprotein Ia* - 155 Kda Platelet Multimerin CLIA kitP-155 CLIA kitP155 CLIA kitMMRN1 CLIA kitECM CLIA kitEMILIN4 CLIA kitGPIA* CLIA kitMMRN CLIA kit |
| Specificity | This kit recognizes Human MMRN1 in samples. No significant cross-reactivity or interference between Human MMRN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-115 | 105 |
| EDTA plasma(n=8) | 101-115 | 107 |
| Cell culture media(n=8) | 86-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.41 | 126.23 | 434.08 | 47.72 | 116.03 | 455.70 |
| Standard deviation | 5.12 | 14.52 | 43.89 | 6.14 | 9.27 | 34.41 |
| CV (%) | 10.80 | 11.50 | 10.11 | 12.87 | 7.99 | 7.55 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
