• Human MMP8 (Neutrophil collagenase) Sandwich ELISA (STJE0002982)
  • Human MMP8 (Neutrophil collagenase) Sandwich ELISA (STJE0002982)

Human MMP8 (Neutrophil collagenase) Sandwich ELISA (STJE0002982)

SKU:
STJE0002982

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This MMP8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MMP8
Gene ID: 4317
Uniprot ID: MMP8_HUMAN
Specificity: This kit recognizes Human MMP-8 in samples. No significant cross-reactivity or interference between Human MMP-8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Can degrade fibrillar type I, II, and III collagens.
Protein Name Neutrophil Collagenase
Matrix Metalloproteinase-8
Mmp-8
Pmnl Collagenase
Pmnl-Cl
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-6798695
Cellular Localisation Cytoplasmic Granule
Secreted
Extracellular Space
Extracellular Matrix
Stored In Intracellular Granules
Alternative ELISA Names Neutrophil Collagenase ELISA kit
Matrix Metalloproteinase-8 ELISA kit
Mmp-8 ELISA kit
Pmnl Collagenase ELISA kit
Pmnl-Cl ELISA kit
MMP8 ELISA kit
CLG1 ELISA kit
Specificity This kit recognizes Human MMP-8 in samples. No significant cross-reactivity or interference between Human MMP-8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-96 91
EDTA plasma (n=8) 89-102 96
Cell culture media (n=8) 95-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.20 4.40 0.50 1.20 4.10
Standard deviation 0.03 0.06 0.23 0.03 0.06 0.17
CV (%) 6.00 5.00 5.23 6.00 5.00 4.15
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance