| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5ng/mL |
| Detection Limit: |
62.5~4000ng/mL |
| Short Description: |
This human MMP25 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MMP25 |
| Gene ID: |
64386 |
| Uniprot ID: |
MMP25_HUMAN |
| Specificity: |
This kit recognizes Human MMP-25 in samples. No significant cross-reactivity or interference between Human MMP-25 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas. |
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. |
| Function | May activate progelatinase A. |
| Protein Name | Matrix Metalloproteinase-25Mmp-25LeukolysinMembrane-Type Matrix Metalloproteinase 6Mt-Mmp 6Mtmmp6Membrane-Type-6 Matrix MetalloproteinaseMt6-MmpMt6mmp |
| Database Links | Reactome: R-HSA-1592389Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular SideSecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Matrix Metalloproteinase-25 CLIA kitMmp-25 CLIA kitLeukolysin CLIA kitMembrane-Type Matrix Metalloproteinase 6 CLIA kitMt-Mmp 6 CLIA kitMtmmp6 CLIA kitMembrane-Type-6 Matrix Metalloproteinase CLIA kitMt6-Mmp CLIA kitMt6mmp CLIA kitMMP25 CLIA kitMMP20 CLIA kitMMPL1 CLIA kitMT6MMP CLIA kit |
| Specificity | This kit recognizes Human MMP-25 in samples. No significant cross-reactivity or interference between Human MMP-25 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-107 | 98 |
| EDTA plasma(n=8) | 87-100 | 94 |
| Cell culture media(n=8) | 98-114 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 216.26 | 357.97 | 1672.60 | 232.55 | 361.96 | 1530.79 |
| Standard deviation | 23.25 | 41.88 | 170.77 | 30.02 | 33.52 | 144.20 |
| CV (%) | 10.75 | 11.70 | 10.21 | 12.91 | 9.26 | 9.42 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
