• Human MMP17 (Matrix metalloproteinase-17) CLIA Kit (STJC0000647)

Human MMP17 (Matrix metalloproteinase-17) CLIA Kit (STJC0000647)

SKU:
STJC0000647

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human MMP17 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: MMP17
Gene ID: 4326
Uniprot ID: MMP17_HUMAN
Specificity: This kit recognizes Human MMP-17 in samples. No significant cross-reactivity or interference between Human MMP-17 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Cleaves pro-TNF-alpha at the '74-Ala-|-Gln-75' site. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
Protein Name Matrix Metalloproteinase-17
Mmp-17
Membrane-Type Matrix Metalloproteinase 4
Mt-Mmp 4
Mtmmp4
Membrane-Type-4 Matrix Metalloproteinase
Mt4-Mmp
Mt4mmp
Database Links Reactome: R-HSA-1592389
Cellular Localisation Isoform Long: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Matrix Metalloproteinase-17 CLIA kit
Mmp-17 CLIA kit
Membrane-Type Matrix Metalloproteinase 4 CLIA kit
Mt-Mmp 4 CLIA kit
Mtmmp4 CLIA kit
Membrane-Type-4 Matrix Metalloproteinase CLIA kit
Mt4-Mmp CLIA kit
Mt4mmp CLIA kit
MMP17 CLIA kit
MT4MMP CLIA kit
Specificity This kit recognizes Human MMP-17 in samples. No significant cross-reactivity or interference between Human MMP-17 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 94
EDTA plasma(n=8) 92-105 98
Cell culture media(n=8) 96-114 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 97.10 299.90 867.30 91.95 282.03 946.19
Standard deviation 12.45 22.85 91.76 8.36 26.26 64.15
CV (%) 12.82 7.62 10.58 9.09 9.31 6.78
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance