| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This human MMP12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MMP12 |
| Gene ID: |
4321 |
| Uniprot ID: |
MMP12_HUMAN |
| Specificity: |
This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Found in alveolar macrophages but not in peripheral blood monocytes. |
| Function | May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. |
| Protein Name | Macrophage MetalloelastaseMmeMacrophage ElastaseMeHmeMatrix Metalloproteinase-12Mmp-12 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Macrophage Metalloelastase CLIA kitMme CLIA kitMacrophage Elastase CLIA kitMe CLIA kitHme CLIA kitMatrix Metalloproteinase-12 CLIA kitMmp-12 CLIA kitMMP12 CLIA kitHME CLIA kit |
| Specificity | This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 94 |
| EDTA plasma(n=8) | 96-113 | 103 |
| Cell culture media(n=8) | 90-101 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 263.96 | 689.48 | 2461.65 | 274.56 | 670.92 | 2684.98 |
| Standard deviation | 28.53 | 80.88 | 149.91 | 33.47 | 63.20 | 202.72 |
| CV (%) | 10.81 | 11.73 | 6.09 | 12.19 | 9.42 | 7.55 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
