| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human MGLL kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MGLL |
| Gene ID: |
11343 |
| Uniprot ID: |
MGLL_HUMAN |
| Specificity: |
This kit recognizes Human MGL in samples. No significant cross-reactivity or interference between Human MGL and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in adipose tissue, lung, liver, kidney, brain and heart. |
| Function | Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. |
| Protein Name | Monoglyceride LipaseMglHu-K5Lysophospholipase HomologLysophospholipase-LikeMonoacylglycerol LipaseMagl |
| Database Links | Reactome: R-HSA-1482883Reactome: R-HSA-163560Reactome: R-HSA-426048 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane Protein |
| Alternative CLIA Names | Monoglyceride Lipase CLIA kitMgl CLIA kitHu-K5 CLIA kitLysophospholipase Homolog CLIA kitLysophospholipase-Like CLIA kitMonoacylglycerol Lipase CLIA kitMagl CLIA kitMGLL CLIA kit |
| Specificity | This kit recognizes Human MGL in samples. No significant cross-reactivity or interference between Human MGL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-104 | 98 |
| EDTA plasma(n=8) | 93-106 | 100 |
| Cell culture media(n=8) | 93-110 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.03 | 290.16 | 822.18 | 86.07 | 280.94 | 743.11 |
| Standard deviation | 7.66 | 23.94 | 71.04 | 8.47 | 32.28 | 74.39 |
| CV (%) | 8.06 | 8.25 | 8.64 | 9.84 | 11.49 | 10.01 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
