| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human MELTF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MELTF |
| Gene ID: |
4241 |
| Uniprot ID: |
TRFM_HUMAN |
| Specificity: |
This kit recognizes Human MFI2 in samples. No significant cross-reactivity or interference between Human MFI2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Found predominantly in human melanomas and in certain fetal tissues.also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts. |
| Function | Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. |
| Protein Name | MelanotransferrinMelanoma-Associated Antigen P97Cd Antigen Cd228 |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Isoform 1: Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative CLIA Names | Melanotransferrin CLIA kitMelanoma-Associated Antigen P97 CLIA kitCd Antigen Cd228 CLIA kitMELTF CLIA kitMAP97 CLIA kitMFI2 CLIA kit |
| Specificity | This kit recognizes Human MFI2 in samples. No significant cross-reactivity or interference between Human MFI2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-109 | 100 |
| EDTA plasma(n=8) | 98-112 | 104 |
| Cell culture media(n=8) | 85-101 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.11 | 117.57 | 485.24 | 51.47 | 127.97 | 446.54 |
| Standard deviation | 5.51 | 12.16 | 31.10 | 6.26 | 13.41 | 38.89 |
| CV (%) | 11.45 | 10.34 | 6.41 | 12.16 | 10.48 | 8.71 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
