| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.105ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This MELK Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
MELK |
| Gene ID: |
9833 |
| Uniprot ID: |
MELK_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in placenta, kidney, thymus, testis, ovary and intestine. |
| Post Translational Modifications | Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable). |
| Function | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity.phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. |
| Protein Name | Maternal Embryonic Leucine Zipper KinaseHmelkProtein Kinase Eg3Peg3 KinaseProtein Kinase Pk38Hpk38Tyrosine-Protein Kinase Melk |
| Database Links | |
| Cellular Localisation | Cell MembranePeripheral Membrane Protein |
| Alternative ELISA Names | Maternal Embryonic Leucine Zipper Kinase ELISA kitHmelk ELISA kitProtein Kinase Eg3 ELISA kitPeg3 Kinase ELISA kitProtein Kinase Pk38 ELISA kitHpk38 ELISA kitTyrosine-Protein Kinase Melk ELISA kitMELK ELISA kitKIAA0175 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
