| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human LTBR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LTBR |
| Gene ID: |
4055 |
| Uniprot ID: |
TNR3_HUMAN |
| Specificity: |
This kit recognizes Human LT Beta R in samples. No significant cross-reactivity or interference between Human LT Beta R and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 3Lymphotoxin-Beta ReceptorTumor Necrosis Factor C ReceptorTumor Necrosis Factor Receptor 2-Related ProteinTumor Necrosis Factor Receptor Type IiiTnf-RiiiTnfr-Iii |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5676594 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Tumor Necrosis Factor Receptor Superfamily Member 3 CLIA kitLymphotoxin-Beta Receptor CLIA kitTumor Necrosis Factor C Receptor CLIA kitTumor Necrosis Factor Receptor 2-Related Protein CLIA kitTumor Necrosis Factor Receptor Type Iii CLIA kitTnf-Riii CLIA kitTnfr-Iii CLIA kitLTBR CLIA kitD12S370 CLIA kitTNFCR CLIA kitTNFR3 CLIA kitTNFRSF3 CLIA kit |
| Specificity | This kit recognizes Human LTβR in samples. No significant cross-reactivity or interference between Human LTβR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-96 | 91 |
| EDTA plasma(n=8) | 99-116 | 108 |
| Cell culture media(n=8) | 86-98 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 195.26 | 403.94 | 1376.58 | 195.96 | 386.93 | 1413.13 |
| Standard deviation | 21.09 | 36.19 | 89.62 | 17.17 | 30.53 | 153.47 |
| CV (%) | 10.80 | 8.96 | 6.51 | 8.76 | 7.89 | 10.86 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
