| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human LTB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LTB |
| Gene ID: |
4050 |
| Uniprot ID: |
TNFC_HUMAN |
| Specificity: |
This kit recognizes Human LT Beta in samples. No significant cross-reactivity or interference between Human LT Beta and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Spleen and thymus. |
| Function | Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. |
| Protein Name | Lymphotoxin-BetaLt-BetaTumor Necrosis Factor CTnf-CTumor Necrosis Factor Ligand Superfamily Member 3 |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5676594 |
| Cellular Localisation | MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative CLIA Names | Lymphotoxin-Beta CLIA kitLt-Beta CLIA kitTumor Necrosis Factor C CLIA kitTnf-C CLIA kitTumor Necrosis Factor Ligand Superfamily Member 3 CLIA kitLTB CLIA kitTNFC CLIA kitTNFSF3 CLIA kit |
| Specificity | This kit recognizes Human LTβ in samples. No significant cross-reactivity or interference between Human LTβ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-100 | 92 |
| EDTA plasma(n=8) | 100-113 | 105 |
| Cell culture media(n=8) | 95-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 27.53 | 41.86 | 246.67 | 28.60 | 38.63 | 228.74 |
| Standard deviation | 2.92 | 4.34 | 25.26 | 2.77 | 2.93 | 15.49 |
| CV (%) | 10.61 | 10.37 | 10.24 | 9.69 | 7.58 | 6.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
