| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.117ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This LEI Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
SERPINB1 |
| Gene ID: |
1992 |
| Uniprot ID: |
ILEU_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes. |
| Post Translational Modifications | |
| Function | Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function. |
| Protein Name | Leukocyte Elastase InhibitorLeiMonocyte/Neutrophil Elastase InhibitorEiM/NeiPeptidase Inhibitor 2Pi-2Serpin B1 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedCytoplasmCytolytic GranuleEarly Endosome |
| Alternative ELISA Names | Leukocyte Elastase Inhibitor ELISA kitLei ELISA kitMonocyte/Neutrophil Elastase Inhibitor ELISA kitEi ELISA kitM/Nei ELISA kitPeptidase Inhibitor 2 ELISA kitPi-2 ELISA kitSerpin B1 ELISA kitSERPINB1 ELISA kitELANH2 ELISA kitMNEI ELISA kitPI2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
