| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.053ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This KRT14 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
KRT14 |
| Gene ID: |
3861 |
| Uniprot ID: |
K1C14_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. |
| Post Translational Modifications | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
| Function | The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. |
| Protein Name | Keratin - Type I Cytoskeletal 14Cytokeratin-14Ck-14Keratin-14K14 |
| Database Links | Reactome: R-HSA-446107Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | CytoplasmNucleusExpressed In Both As A Filamentous Pattern |
| Alternative ELISA Names | Keratin - Type I Cytoskeletal 14 ELISA kitCytokeratin-14 ELISA kitCk-14 ELISA kitKeratin-14 ELISA kitK14 ELISA kitKRT14 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
