• Human KLF4 (Kruppel Like Factor 4, Gut) Sandwich ELISA Kit (STJE0010179)

Human KLF4 (Kruppel Like Factor 4, Gut) Sandwich ELISA Kit (STJE0010179)

SKU:
STJE0010179

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.060ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This KLF4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: KLF4
Gene ID: 9314
Uniprot ID: KLF4_HUMAN
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6.deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.
Function Transcription factor.can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Protein Name Krueppel-Like Factor 4
Epithelial Zinc Finger Protein Ezf
Gut-Enriched Krueppel-Like Factor
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-422085
Reactome: R-HSA-452723
Reactome: R-HSA-9617828
Cellular Localisation Nucleus
Cytoplasm
Alternative ELISA Names Krueppel-Like Factor 4 ELISA kit
Epithelial Zinc Finger Protein Ezf ELISA kit
Gut-Enriched Krueppel-Like Factor ELISA kit
KLF4 ELISA kit
EZF ELISA kit
GKLF ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance