• Human KITLG (Kit ligand) Sandwich ELISA (STJE0002866)

Human KITLG (Kit ligand) Sandwich ELISA (STJE0002866)

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STJE0002866

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This KITLG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: KITLG
Gene ID: 4254
Uniprot ID: SCF_HUMAN
Specificity: This kit recognizes Human SCF in samples. No significant cross-reactivity or interference between Human SCF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Protein Name Kit Ligand
Mast Cell Growth Factor
Mgf
Stem Cell Factor
Scf
C-Kit Ligand Cleaved Into - Soluble Kit Ligand
Skitlg
Database Links Reactome: R-HSA-1257604 P21583-1
Reactome: R-HSA-1433557 P21583-1
Reactome: R-HSA-1433559 P21583-1
Reactome: R-HSA-2219530 P21583-1
Reactome: R-HSA-5673001 P21583-1
Reactome: R-HSA-6811558 P21583-1
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cytoplasm
Cytoplasm
Cytoskeleton
Cell Membrane
Cell Projection
Lamellipodium
Filopodium
Soluble Kit Ligand: Secreted
Alternative ELISA Names Kit Ligand ELISA kit
Mast Cell Growth Factor ELISA kit
Mgf ELISA kit
Stem Cell Factor ELISA kit
Scf ELISA kit
C-Kit Ligand Cleaved Into - Soluble Kit Ligand ELISA kit
Skitlg ELISA kit
KITLG ELISA kit
MGF ELISA kit
SCF ELISA kit
Specificity This kit recognizes Human SCF in samples. No significant cross-reactivity or interference between Human SCF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 93
EDTA plasma (n=8) 92-104 97
Cell culture media (n=8) 96-110 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 109.58 274.64 883.91 116.10 293.04 841.62
Standard deviation 5.82 11.81 44.64 7.83 13.48 25.33
CV (%) 5.31 4.30 5.05 6.74 4.60 3.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance