| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This KISS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
KISS1 |
| Gene ID: |
3814 |
| Uniprot ID: |
KISS1_HUMAN |
| Specificity: |
This kit recognizes Human KISS1 in samples. No significant cross-reactivity or interference between Human KISS1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Processed by MMP2 and MMP9. |
| Function | Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. |
| Protein Name | Metastasis-Suppressor Kiss-1Kisspeptin-1 Cleaved Into - MetastinKisspeptin-54 - Kisspeptin-14 - Kisspeptin-13 - Kisspeptin-10 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Metastasis-Suppressor Kiss-1 ELISA kitKisspeptin-1 Cleaved Into - Metastin ELISA kitKisspeptin-54 - Kisspeptin-14 - Kisspeptin-13 - Kisspeptin-10 ELISA kitKISS1 ELISA kitPP5098 ELISA kit |
| Specificity | This kit recognizes Human KISS1 in samples. No significant cross-reactivity or interference between Human KISS1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-97 | 92 |
| EDTA plasma (n=8) | 87-98 | 91 |
| Cell culture media (n=8) | 88-95 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 93.38 | 219.25 | 939.15 | 88.93 | 224.16 | 958.91 |
| Standard deviation | 5.49 | 10.02 | 40.67 | 5.23 | 10.98 | 38.45 |
| CV (%) | 5.88 | 4.57 | 4.33 | 5.88 | 4.90 | 4.01 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
