| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.128ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This KDM4A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
KDM4A |
| Gene ID: |
9682 |
| Uniprot ID: |
KDM4A_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited. |
| Function | Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. |
| Protein Name | Lysine-Specific Demethylase 4aJmjc Domain-Containing Histone Demethylation Protein 3aJumonji Domain-Containing Protein 2aHistone H3-Trimethyl-L-Lysine(36 Demethylase 4aHistone H3-Trimethyl-L-Lysine(9 Demethylase 4a |
| Database Links | Reactome: R-HSA-3214842Reactome: R-HSA-5693565Reactome: R-HSA-9029569 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Lysine-Specific Demethylase 4a ELISA kitJmjc Domain-Containing Histone Demethylation Protein 3a ELISA kitJumonji Domain-Containing Protein 2a ELISA kitHistone H3-Trimethyl-L-Lysine(36 Demethylase 4a ELISA kitHistone H3-Trimethyl-L-Lysine(9 Demethylase 4a ELISA kitKDM4A ELISA kitJHDM3A ELISA kitJMJD2 ELISA kitJMJD2A ELISA kitKIAA0677 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
