| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | JAM3 |
| Gene ID: | 83700 |
| Uniprot ID: | JAM3_HUMAN |
| Immunogen Region: | Val32-Asn241 Ala149Pro |
| Immunogen: | Recombinant Human JAM-3 (A149P) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val32-Asn241 (Ala149Pro) ) of human JAM3/JAM-C (Accession #NP_116190.3) fused with an Fc, 6×His tag at the C-terminu |
| Immunogen Sequence: | VNLKSSNRTPVVQEFESVEL SCIITDSQTSDPRIEWKKIQ DEQTTYVFFDNKIQGDLAGR AEILGKTSLKIWNVTRRDSA LYRCEVVARNDRKEIDEIVI ELTVQVKPVTPVCRVPKPVP VGKMATLHCQESEGHPRPHY SWYRNDVPLPTDSRANPRFR NSSFHLNSETGTLVFTAVHK DDSGQYYCIASNDAGSARCE EQEMEVYDLN |
| Tissue Specificity | Detected on round and elongated spermatids (at protein level). Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level). |
| Post Translational Modifications | Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17.the release of soluble JAM3 is increased by pro-inflammatory factors. S-palmitoylated by ZDHHC7. S-palmitoylation promotes expression at tight junctions. |
| Function | Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium. Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation. Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). Plays a role in angiogenesis. Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion. Soluble form of JAM-C: Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis. |
| Protein Name | Junctional Adhesion Molecule C Jam-C Jam-2 Junctional Adhesion Molecule 3 Jam-3 Cleaved Into - Soluble Form Of Jam-C Sjam-C |
| Database Links | Reactome: R-HSA-202733 Reactome: R-HSA-216083 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Junction Desmosome Tight Junction Detected In The Acrosome Region In Developing Spermatids In Epithelial Cells It Is Expressed At Desmosomes But Not At Tight Junctions Localizes At The Cell Surface Of Endothelial Cells Treatment Of Endothelial Cells With Vascular Endothelial Growth Factor Stimulates Recruitment Of Jam3 To Cell-Cell Contacts Soluble Form Of Jam-C: Secreted |
| Alternative Protein Names | Junctional Adhesion Molecule C protein Jam-C protein Jam-2 protein Junctional Adhesion Molecule 3 protein Jam-3 Cleaved Into - Soluble Form Of Jam-C protein Sjam-C protein JAM3 protein UNQ859 protein PRO1868 protein |
Information sourced from Uniprot.org