| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.53ng/mL |
| Detection Limit: |
1.56-100ng/mL |
| Short Description: |
This IRAK4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
IRAK4 |
| Gene ID: |
51135 |
| Uniprot ID: |
IRAK4_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylated. |
| Function | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| Protein Name | Interleukin-1 Receptor-Associated Kinase 4Irak-4Renal Carcinoma Antigen Ny-Ren-64 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-166058Reactome: R-HSA-5603037Reactome: R-HSA-5603041Reactome: R-HSA-6811558Reactome: R-HSA-9020702Reactome: R-HSA-975110Reactome: R-HSA-975138Reactome: R-HSA-975155Reactome: R-HSA-975871 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Interleukin-1 Receptor-Associated Kinase 4 ELISA kitIrak-4 ELISA kitRenal Carcinoma Antigen Ny-Ren-64 ELISA kitIRAK4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
