| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
32pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This iPLA2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
PLA2G6 |
| Gene ID: |
8398 |
| Uniprot ID: |
PLPL9_HUMAN |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | Four different transcripts were found to be expressed in a distinct tissue distribution. |
| Post Translational Modifications | |
| Function | Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion. Isoform Ankyrin-iPLA2-1: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. |
| Protein Name | 85/88 Kda Calcium-Independent Phospholipase A2Cai-Pla22-Lysophosphatidylcholine AcylhydrolaseGroup Vi Phospholipase A2Gvi Pla2Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 BetaIpla2-BetaPalmitoyl-Coa HydrolasePatatin-Like Phospholipase Domain-Containing Protein 9Pnpla9 |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482798Reactome: R-HSA-1482839Reactome: R-HSA-2029485Reactome: R-HSA-6811436 |
| Cellular Localisation | CytoplasmCell MembraneMitochondrionCell ProjectionPseudopodiumRecruited To The Membrane-Enriched Pseudopods Upon Mcp1/Ccl2 Stimulation In Monocytes |
| Alternative ELISA Names | 85/88 Kda Calcium-Independent Phospholipase A2 ELISA kitCai-Pla2 ELISA kit2-Lysophosphatidylcholine Acylhydrolase ELISA kitGroup Vi Phospholipase A2 ELISA kitGvi Pla2 ELISA kitIntracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta ELISA kitIpla2-Beta ELISA kitPalmitoyl-Coa Hydrolase ELISA kitPatatin-Like Phospholipase Domain-Containing Protein 9 ELISA kitPnpla9 ELISA kitPLA2G6 ELISA kitPLPLA9 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
