• Human INSL5 (Insulin-like peptide INSL5) CLIA Kit (STJC0000241)

Human INSL5 (Insulin-like peptide INSL5) CLIA Kit (STJC0000241)

SKU:
STJC0000241

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human INSL5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: INSL5
Gene ID: 10022
Uniprot ID: INSL5_HUMAN
Specificity: This kit recognizes Human INSL5 in samples. No significant cross-reactivity or interference between Human INSL5 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in rectum with lower levels in uterus and ascending and descending colon.
Function May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
Protein Name Insulin-Like Peptide Insl5
Insulin-Like Peptide 5 Cleaved Into - Insulin-Like Peptide Insl5 B Chain - Insulin-Like Peptide Insl5 A Chain
Database Links Reactome: R-HSA-418594
Reactome: R-HSA-444821
Cellular Localisation Secreted
Alternative CLIA Names Insulin-Like Peptide Insl5 CLIA kit
Insulin-Like Peptide 5 Cleaved Into - Insulin-Like Peptide Insl5 B Chain - Insulin-Like Peptide Insl5 A Chain CLIA kit
INSL5 CLIA kit
UNQ156 CLIA kit
PRO182 CLIA kit
Specificity This kit recognizes Human INSL5 in samples. No significant cross-reactivity or interference between Human INSL5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 101-115 108
EDTA plasma(n=8) 87-102 93
Cell culture media(n=8) 87-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 102.94 303.87 796.74 109.20 327.90 718.54
Standard deviation 9.66 34.58 78.48 13.87 23.87 59.35
CV (%) 9.38 11.38 9.85 12.70 7.28 8.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance