• Human INSL3 (Insulin-like 3) Sandwich ELISA (STJE0002200)

Human INSL3 (Insulin-like 3) Sandwich ELISA (STJE0002200)

SKU:
STJE0002200

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This INSL3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: INSL3
Gene ID: 3640
Uniprot ID: INSL3_HUMAN
Specificity: This kit recognizes Human INSL3 in samples. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
Protein Name Insulin-Like 3
Leydig Insulin-Like Peptide
Ley-I-L
Relaxin-Like Factor Cleaved Into - Insulin-Like 3 B Chain - Insulin-Like 3 A Chain
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-444821
Cellular Localisation Secreted
Alternative ELISA Names Insulin-Like 3 ELISA kit
Leydig Insulin-Like Peptide ELISA kit
Ley-I-L ELISA kit
Relaxin-Like Factor Cleaved Into - Insulin-Like 3 B Chain - Insulin-Like 3 A Chain ELISA kit
INSL3 ELISA kit
RLF ELISA kit
RLNL ELISA kit
Specificity This kit recognizes Human INSL3 in samples. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-109 100
EDTA plasma (n=8) 94-107 99
Cell culture media (n=8) 94-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.05 4.92 0.44 1.06 4.50
Standard deviation 0.03 0.06 0.17 0.03 0.05 0.22
CV (%) 6.38 5.71 3.46 6.82 4.72 4.89
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance