• Human INS (Insulin) Sandwich ELISA (STJE0003795)
  • Human INS (Insulin) Sandwich ELISA (STJE0003795)

Human INS (Insulin) Sandwich ELISA (STJE0003795)

SKU:
STJE0003795

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47 Mu IU/mL
Detection Limit: 0.78~50 Mu IU/mL
Short Description: This INS Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: INS
Gene ID: 3630
Uniprot ID: INS_HUMAN
Specificity: This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Insulin Cleaved Into - Insulin B Chain - Insulin A Chain
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-264876
Reactome: R-HSA-422085
Reactome: R-HSA-422356
Reactome: R-HSA-6807878
Reactome: R-HSA-6811558
Reactome: R-HSA-74713
Reactome: R-HSA-74749
Reactome: R-HSA-74751
Reactome: R-HSA-74752
Reactome: R-HSA-77387
Reactome: R-HSA-9615017
Reactome: R-HSA-9768919
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative ELISA Names Insulin Cleaved Into - Insulin B Chain - Insulin A Chain ELISA kit
INS ELISA kit
Specificity This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 94
EDTA plasma (n=8) 96-108 101
Cell culture media (n=8) 94-106 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (μIU/mL) 2.38 5.03 23.63 2.21 6.19 21.47
Standard deviation 0.13 0.25 1.25 0.11 0.29 0.97
CV (%) 5.46 4.97 5.29 4.98 4.68 4.52
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance