| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
3.6pg/mL |
| Detection Limit: |
7.81-500pg/mL |
| Short Description: |
This IL8 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
CXCL8 |
| Gene ID: |
3576 |
| Uniprot ID: |
IL8_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP.leading to impaired neutrophil endothelial transmigration and thus increased virulence. |
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8Il-8C-X-C Motif Chemokine 8ChemokineC-X-C Motif Ligand 8EmoctakinGranulocyte Chemotactic Protein 1Gcp-1Monocyte-Derived Neutrophil Chemotactic FactorMdncfMonocyte-Derived Neutrophil-Activating PeptideMonapNeutrophil-Activating Protein 1Nap-1Protein 3-10cT-Cell Chemotactic Factor Cleaved Into - Mdncf-AGcp/Il-8 Protein IvIl8/Nap1 Form I - Interleukin-8(Ala-Il-877Gcp/Il-8 Protein IiIl-8(1-77Il8/Nap1 Form IiMdncf-B - Il-8(5-77 - Il-8(6-77(Ser-Il-872Gcp/Il-8 Protein IIl8/Nap1 Form IiiLymphocyte-Derived Neutrophil-Activating FactorLynapMdncf-CNeutrophil-Activating FactorNaf - Il-8(7-77Gcp/Il-8 Protein VIl8/Nap1 Form Iv - Il-8(8-77Gcp/Il-8 Protein ViIl8/Nap1 Form V - Il-8(9-77Gcp/Il-8 Protein IiiIl8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-375276Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-418594Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Interleukin-8 ELISA kitIl-8 ELISA kitC-X-C Motif Chemokine 8 ELISA kitChemokine ELISA kitC-X-C Motif Ligand 8 ELISA kitEmoctakin ELISA kitGranulocyte Chemotactic Protein 1 ELISA kitGcp-1 ELISA kitMonocyte-Derived Neutrophil Chemotactic Factor ELISA kitMdncf ELISA kitMonocyte-Derived Neutrophil-Activating Peptide ELISA kitMonap ELISA kitNeutrophil-Activating Protein 1 ELISA kitNap-1 ELISA kitProtein 3-10c ELISA kitT-Cell Chemotactic Factor Cleaved Into - Mdncf-A ELISA kitGcp/Il-8 Protein Iv ELISA kitIl8/Nap1 Form I - Interleukin-8 ELISA kit(Ala-Il-877 ELISA kitGcp/Il-8 Protein Ii ELISA kitIl-8(1-77 ELISA kitIl8/Nap1 Form Ii ELISA kitMdncf-B - Il-8(5-77 - Il-8(6-77 ELISA kit(Ser-Il-872 ELISA kitGcp/Il-8 Protein I ELISA kitIl8/Nap1 Form Iii ELISA kitLymphocyte-Derived Neutrophil-Activating Factor ELISA kitLynap ELISA kitMdncf-C ELISA kitNeutrophil-Activating Factor ELISA kitNaf - Il-8(7-77 ELISA kitGcp/Il-8 Protein V ELISA kitIl8/Nap1 Form Iv - Il-8(8-77 ELISA kitGcp/Il-8 Protein Vi ELISA kitIl8/Nap1 Form V - Il-8(9-77 ELISA kitGcp/Il-8 Protein Iii ELISA kitIl8/Nap1 Form Vi ELISA kitCXCL8 ELISA kitIL8 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
