| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
5.8pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This IL4R Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
IL4R |
| Gene ID: |
3566 |
| Uniprot ID: |
IL4RA_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Isoform 1 and isoform 2 are highly expressed in activated T-cells. |
| Post Translational Modifications | On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction. The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase. |
| Function | Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells. |
| Protein Name | Interleukin-4 Receptor Subunit AlphaIl-4 Receptor Subunit AlphaIl-4r Subunit AlphaIl-4r-AlphaIl-4raCd Antigen Cd124 Cleaved Into - Soluble Interleukin-4 Receptor Subunit AlphaSoluble Il-4 Receptor Subunit AlphaSoluble Il-4r-AlphaSil4ralpha/ProtIl-4-Binding ProteinIl4-Bp |
| Database Links | Reactome: R-HSA-6785807 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative ELISA Names | Interleukin-4 Receptor Subunit Alpha ELISA kitIl-4 Receptor Subunit Alpha ELISA kitIl-4r Subunit Alpha ELISA kitIl-4r-Alpha ELISA kitIl-4ra ELISA kitCd Antigen Cd124 Cleaved Into - Soluble Interleukin-4 Receptor Subunit Alpha ELISA kitSoluble Il-4 Receptor Subunit Alpha ELISA kitSoluble Il-4r-Alpha ELISA kitSil4ralpha/Prot ELISA kitIl-4-Binding Protein ELISA kitIl4-Bp ELISA kitIL4R ELISA kitIL4RA ELISA kit582J2.1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
