• Human IL1A (IL1A) Sandwich ELISA Kit (STJE0000019)

Human IL1A (IL1A) Sandwich ELISA Kit (STJE0000019)

SKU:
STJE0000019-96

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 4 pg/mL
Detection Limit: 78-500 pg/ml
Short Description: This IL1A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Conjugation: Biotin
Storage Instruction: Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Assay Time: 4 hours
Detection: Colorimetric
Gene Symbol: IL1A
Gene ID: 3552
Uniprot ID: IL1A_HUMAN
Sample Type: serum, plasma and cell culture supernates
Tissue Specificity
Post Translational Modifications Acetylated within its nuclear localization sequence, which impacts subcellular localization. Proteolytic processed by CAPN1 in a calcium-dependent manner. Cleavage from 31 kDa precursor to 18 kDa biologically active molecules. Phosphorylated. Phosphorylation greatly enhances susceptibility to digestion and promotes the conversion of pre-IL1A alpha to the biologically active IL1A.
Function Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.
Protein Name Interleukin-1 Alpha
Il-1 Alpha
Hematopoietin-1
Database Links Reactome: R-HSA-2559582
Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9020702
Reactome: R-HSA-9660826
Cellular Localisation Nucleus
Cytoplasm
Secreted
The Lack Of A Specific Hydrophobic Segment In The Precursor Sequence Suggests That Il-1 Is Released By Damaged Cells Or Is Secreted By A Mechanism Differing From That Used For Other Secretory Proteins
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Recruited To Dna Damage Sites And Secreted After Genotoxic Stress
Alternative ELISA Names Interleukin-1 Alpha ELISA kit
Il-1 Alpha ELISA kit
Hematopoietin-1 ELISA kit
IL1A ELISA kit
IL1F1 ELISA kit
output

Information sourced from Uniprot.org

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