| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human IL16 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
IL16 |
| Gene ID: |
3603 |
| Uniprot ID: |
IL16_HUMAN |
| Specificity: |
This kit recognizes Human IL-16 in samples. No significant cross-reactivity or interference between Human IL-16 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 3: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation. |
| Post Translational Modifications | Isoform 3: Synthesized as a chemo-attractant inactive precursor in hemopoietic tissues, and proteolytically cleaved by caspase-3 to yield IL-16. |
| Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1: May act as a scaffolding protein that anchors ion channels in the membrane. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. |
| Protein Name | Pro-Interleukin-16 Cleaved Into - Interleukin-16Il-16Lymphocyte Chemoattractant FactorLcf |
| Database Links | Reactome: R-HSA-449836 |
| Cellular Localisation | Interleukin-16: SecretedIsoform 1: CytoplasmIsoform 3: CytoplasmNucleus |
| Alternative CLIA Names | Pro-Interleukin-16 Cleaved Into - Interleukin-16 CLIA kitIl-16 CLIA kitLymphocyte Chemoattractant Factor CLIA kitLcf CLIA kitIL16 CLIA kit |
| Specificity | This kit recognizes Human IL-16 in samples. No significant cross-reactivity or interference between Human IL-16 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-113 | 106 |
| EDTA plasma(n=8) | 97-115 | 105 |
| Cell culture media(n=8) | 98-112 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.53 | 118.16 | 319.07 | 40.58 | 124.15 | 346.73 |
| Standard deviation | 3.90 | 10.81 | 31.46 | 4.10 | 13.68 | 32.94 |
| CV (%) | 10.39 | 9.15 | 9.86 | 10.10 | 11.02 | 9.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
