• Human IGFBP3 (Insulin-like growth factor-binding protein 3) CLIA Kit (STJC0000080)

Human IGFBP3 (Insulin-like growth factor-binding protein 3) CLIA Kit (STJC0000080)

SKU:
STJC0000080

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This human IGFBP3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IGFBP3
Gene ID: 3486
Uniprot ID: IBP3_HUMAN
Specificity: This kit recognizes Human IGFBP-3 in samples. No significant cross-reactivity or interference between Human IGFBP-3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed by most tissues. Present in plasma.
Post Translational Modifications Phosphorylated by FAM20C in the extracellular medium.
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis.
Protein Name Insulin-Like Growth Factor-Binding Protein 3
Ibp-3
Igf-Binding Protein 3
Igfbp-3
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-6803211
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative CLIA Names Insulin-Like Growth Factor-Binding Protein 3 CLIA kit
Ibp-3 CLIA kit
Igf-Binding Protein 3 CLIA kit
Igfbp-3 CLIA kit
IGFBP3 CLIA kit
IBP3 CLIA kit
Specificity This kit recognizes Human IGFBP-3 in samples. No significant cross-reactivity or interference between Human IGFBP-3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 94
EDTA plasma(n=8) 97-109 103
Cell culture media(n=8) 88-100 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.02 4.85 0.42 1.07 4.71
Standard deviation 0.06 0.10 0.52 0.05 0.11 0.37
CV (%) 13.04 9.80 10.72 11.90 10.28 7.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance