• Human IGFALS (Insulin-like growth factor-binding protein complex acid labile subunit) CLIA Kit (STJC0000258)

Human IGFALS (Insulin-like growth factor-binding protein complex acid labile subunit) CLIA Kit (STJC0000258)

SKU:
STJC0000258

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This human IGFALS kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IGFALS
Gene ID: 3483
Uniprot ID: ALS_HUMAN
Specificity: This kit recognizes Human IGFALS in samples. No significant cross-reactivity or interference between Human IGFALS and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Plasma.
Function Involved in protein-protein interactions that result in protein complexes, receptor-ligand binding or cell adhesion.
Protein Name Insulin-Like Growth Factor-Binding Protein Complex Acid Labile Subunit
Als
Database Links Reactome: R-HSA-381426
Cellular Localisation Secreted
Extracellular Space
Alternative CLIA Names Insulin-Like Growth Factor-Binding Protein Complex Acid Labile Subunit CLIA kit
Als CLIA kit
IGFALS CLIA kit
ALS CLIA kit
Specificity This kit recognizes Human IGFALS in samples. No significant cross-reactivity or interference between Human IGFALS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-104 95
EDTA plasma(n=8) 100-115 108
Cell culture media(n=8) 85-96 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.00 3.15 9.19 0.90 3.34 8.60
Standard deviation 0.13 0.28 1.02 0.08 0.36 0.93
CV (%) 13.00 8.89 11.10 8.89 10.78 10.81
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance