• Human IFNG (IFNG) Sandwich ELISA Kit (STJE0000010)

Human IFNG (IFNG) Sandwich ELISA Kit (STJE0000010)

SKU:
STJE0000010-96

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 7.3 pg/mL
Detection Limit: 156-1000 pg/mL
Short Description: This IFNG Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Conjugation: Biotin
Storage Instruction: Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Assay Time: 4 hours
Detection: Colorimetric
Gene Symbol: IFNG
Gene ID: 3458
Uniprot ID: IFNG_HUMAN
Sample Type: serum, plasma, cell culture supernates
Tissue Specificity Released primarily from activated T lymphocytes.
Post Translational Modifications Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Function Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
Protein Name Interferon Gamma
Ifn-Gamma
Immune Interferon
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-877312
Reactome: R-HSA-8877330
Reactome: R-HSA-8950505
Reactome: R-HSA-9732724
Cellular Localisation Secreted
Alternative ELISA Names Interferon Gamma ELISA kit
Ifn-Gamma ELISA kit
Immune Interferon ELISA kit
IFNG ELISA kit
output

Information sourced from Uniprot.org

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