| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This IDO1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IDO1 |
| Gene ID: |
3620 |
| Uniprot ID: |
I23O1_HUMAN |
| Specificity: |
This kit recognizes Human IDO in samples. No significant cross-reactivity or interference between Human IDO and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection. |
| Protein Name | Indoleamine 2 -3-Dioxygenase 1Ido-1Indoleamine-Pyrrole 2 -3-Dioxygenase |
| Database Links | Reactome: R-HSA-71240 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative ELISA Names | Indoleamine 2 -3-Dioxygenase 1 ELISA kitIdo-1 ELISA kitIndoleamine-Pyrrole 2 -3-Dioxygenase ELISA kitIDO1 ELISA kitIDO ELISA kitINDO ELISA kit |
| Specificity | This kit recognizes Human IDO in samples. No significant cross-reactivity or interference between Human IDO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-101 | 95 |
| EDTA plasma (n=8) | 86-100 | 92 |
| Cell culture media (n=8) | 92-108 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.95 | 1.81 | 7.37 | 1.03 | 1.96 | 7.88 |
| Standard deviation | 0.06 | 0.10 | 0.23 | 0.07 | 0.12 | 0.41 |
| CV (%) | 6.32 | 5.52 | 3.12 | 6.80 | 6.12 | 5.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
