| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human ICAM4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ICAM4 |
| Gene ID: |
3386 |
| Uniprot ID: |
ICAM4_HUMAN |
| Specificity: |
This kit recognizes Human ICAM4 in samples. No significant cross-reactivity or interference between Human ICAM4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Erythrocytes. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. |
| Protein Name | Intercellular Adhesion Molecule 4Icam-4Landsteiner-Wiener Blood Group GlycoproteinLw Blood Group ProteinCd Antigen Cd242 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083 |
| Cellular Localisation | Isoform Long: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Short: SecretedCell Membrane |
| Alternative CLIA Names | Intercellular Adhesion Molecule 4 CLIA kitIcam-4 CLIA kitLandsteiner-Wiener Blood Group Glycoprotein CLIA kitLw Blood Group Protein CLIA kitCd Antigen Cd242 CLIA kitICAM4 CLIA kitLW CLIA kit |
| Specificity | This kit recognizes Human ICAM4 in samples. No significant cross-reactivity or interference between Human ICAM4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-111 | 101 |
| EDTA plasma(n=8) | 86-101 | 93 |
| Cell culture media(n=8) | 92-104 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.04 | 3.79 | 18.22 | 2.17 | 3.71 | 17.70 |
| Standard deviation | 0.26 | 0.28 | 1.86 | 0.18 | 0.41 | 1.79 |
| CV (%) | 12.75 | 7.39 | 10.21 | 8.29 | 11.05 | 10.11 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
