| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.12 ng/mL |
| Detection Limit: |
0.31-20 ng/mL |
| Short Description: |
This ICAM-3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ICAM3 |
| Gene ID: |
3385 |
| Uniprot ID: |
ICAM3_HUMAN |
| Specificity: |
This kit recognizes Human ICAM-3 in samples. No significant cross-reactivity or interference between Human ICAM-3 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Leukocytes. |
| Post Translational Modifications | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated.glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages. |
| Protein Name | Intercellular Adhesion Molecule 3Icam-3Cdw50Icam-RCd Antigen Cd50 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-5621575 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Intercellular Adhesion Molecule 3 ELISA kitIcam-3 ELISA kitCdw50 ELISA kitIcam-R ELISA kitCd Antigen Cd50 ELISA kitICAM3 ELISA kit |
| Specificity | This kit recognizes Human ICAM-3 in samples. No significant cross-reactivity or interference between Human ICAM-3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 94 |
| EDTA plasma (n=8) | 93-110 | 101 |
| Cell culture media (n=8) | 90-102 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.04 | 2.63 | 9.29 | 1.08 | 2.40 | 9.10 |
| Standard deviation | 0.07 | 0.14 | 0.40 | 0.06 | 0.11 | 0.44 |
| CV (%) | 6.81 | 5.50 | 4.34 | 5.10 | 4.66 | 4.82 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
