Human ICAM-1 (intercellular adhesion molecule 1) Sandwich ELISA (STJE0019990)

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STJE0019990

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.15 ng/mL
Detection Limit: 0.25-16 ng/mL
Short Description: This ICAM-1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 1.5h
Detection: Colormetric
Gene Symbol: ICAM1
Gene ID: 3383
Uniprot ID: ICAM1_HUMAN
Specificity: This kit recognizes Human ICAM-1 in samples. No significant cross-reactivity or interference between Human ICAM-1 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Protein Name Intercellular Adhesion Molecule 1
Icam-1
Major Group Rhinovirus Receptor
Cd Antigen Cd54
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-216083
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-877300
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Intercellular Adhesion Molecule 1 ELISA kit
Icam-1 ELISA kit
Major Group Rhinovirus Receptor ELISA kit
Cd Antigen Cd54 ELISA kit
ICAM1 ELISA kit
Specificity This kit recognizes ICAM-1 in samples. No significant cross-reactivity or interference between ICAM-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T 2-8℃, 1 months
Reference Standard 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×) 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL 2-8℃ (Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×) 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃ (Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-104 96
EDTA plasma(n=8) 87-99 94
Cell culture media(n=8) 91-104 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.86 2.24 5.52 0.87 2.28 5.82
Standard deviation 0.05 0.09 0.25 0.06 0.10 0.19
CV (%) 5.97 4.11 4.47 6.35 4.30 3.18
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance