Human IAPP (Islet amyloid polypeptide) Competitive ELISA (STJE0002128)

SKU:
STJE0002128

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This IAPP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: IAPP
Gene ID: 3375
Uniprot ID: IAPP_HUMAN
Specificity: This kit recognizes Human IAPP in samples. No significant cross-reactivity or interference between Human IAPP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
Protein Name Islet Amyloid Polypeptide
Amylin
Diabetes-Associated Peptide
Dap
Insulinoma Amyloid Peptide
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-418555
Reactome: R-HSA-419812
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative ELISA Names Islet Amyloid Polypeptide ELISA kit
Amylin ELISA kit
Diabetes-Associated Peptide ELISA kit
Dap ELISA kit
Insulinoma Amyloid Peptide ELISA kit
IAPP ELISA kit
Specificity This kit recognizes IAPP in samples. No significant cross-reactivity or interference between IAPP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 92
EDTA plasma(n=8) 85-96 91
Cell culture media(n=8) 95-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 193.90 485.90 1754.10 197.00 478.40 1764.50
Standard deviation 9.90 21.90 84.20 12.60 21.00 84.70
CV (%) 5.11 4.51 4.80 6.40 4.39 4.80
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance