| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This HTRA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
HTRA1 |
| Gene ID: |
5654 |
| Uniprot ID: |
HTRA1_HUMAN |
| Specificity: |
This kit recognizes Human HTRA1 in samples. No significant cross-reactivity or interference between Human HTRA1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. |
| Protein Name | Serine Protease Htra1High-Temperature Requirement A Serine Peptidase 1L56Serine Protease 11 |
| Database Links | Reactome: R-HSA-1474228 |
| Cellular Localisation | Cell MembraneSecretedCytoplasmCytosolPredominantly SecretedAlso Found Associated With The Plasma Membrane |
| Alternative ELISA Names | Serine Protease Htra1 ELISA kitHigh-Temperature Requirement A Serine Peptidase 1 ELISA kitL56 ELISA kitSerine Protease 11 ELISA kitHTRA1 ELISA kitHTRA ELISA kitPRSS11 ELISA kit |
| Specificity | This kit recognizes Human HTRA1 in samples. No significant cross-reactivity or interference between Human HTRA1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-106 | 100 |
| EDTA plasma (n=8) | 93-104 | 99 |
| Cell culture media (n=8) | 85-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.92 | 2.44 | 7.64 | 0.92 | 2.30 | 6.99 |
| Standard deviation | 0.05 | 0.12 | 0.24 | 0.05 | 0.11 | 0.26 |
| CV (%) | 5.43 | 4.92 | 3.14 | 5.43 | 4.78 | 3.72 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
