| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.062ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This HTN3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human saliva, tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
HTN3 |
| Gene ID: |
3347 |
| Uniprot ID: |
HIS3_HUMAN |
| Sample Type: |
saliva, tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | 24 proteolytic products are found in saliva. |
| Function | Histatins are cationic and histidine-rich peptides mainly found in the saliva of higher primates. They are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). Hsts can be divided into two major groups according to their biological functions: antimicrobial Hsts (e.g. Hst 5/HTN3) and cell-activating Hsts (e.g. Hst 1/HTN1, Hst 2/HTN1 and Hst 3/HTN3). Histatin-3: Histatin 3 (Hst 3) is mostly involved in cell migration and wound healing in the oral cavity. Also stimulates cell proliferation after binding to heat shock protein HSC70, which enhances HSC70-CDKN1B complex formation and subsequent ubiquitination during G1/S transition. Also displays antifungal activity against pathogenic yeast Candida albicans, however with less effectiveness than Hst 5. His3-(20-43)-peptide: Histatin 5 (Hst 5), a fragment of Hst 3, is the major histatin exhibiting antifungal and antibacterial activities. It is effective against pathogenic yeast C. albicans, C. neoformans, C. glabrata and S. cerevisiae as well as ESKAPE bacterial pathogens. Secreted Hst 5 mediates a multi-step intracellular mechanism of action against the pathogen. Depending on peptide concentration and pathogen, uptake across the membrane can occur through transporters, direct interaction with plasma membrane and/or receptor-mediated endocytosis. Binds C. albicans cell wall proteins SSA1 and SSA2 and glycans in an energy-independent manner, then is taken up by the cells through fungal polyamine transporters DUR3 and DUR31 in an energy-dependent manner. Internalized Hst5 is then targeted to the energized mitochondrion to induce reactive oxygen species (ROS) formation and subsequent release of intracellular non-lytic ATP which ultimately leads to fungal cell death. In addition, inhibits C. albicans TRK1 potassium-transporter which causes exudation of intracellular K(+), generating an osmotic imbalance leading to delayed membrane lysis and cell death. Also acts as a potent inhibitor of bacterial proteases such as Lys-gingipain and Arg-gingipain (rgpB) from P. gingivalis as well as human metalloproteases MMP2 and MMP9. The binding of metals such as zinc, copper or nickel with Hst 5 results in the protection of the enamel and antimicrobial activities such as the inhibition of microbial growth by decreasing the metal concentration, the formation of ROS commonly associated with redox-active metals, the induction of membrane disruption mediated by zinc binding. Also involved in coating oral surfaces in the form of a salivary film which reduces colonization by C. albicans on epithelial cell surfaces. Secreted Hst 5 can also internalize mammalian epithelial cells and target the mitochondria although it does not exert cytotoxic effects in these cells. In contrast with Hst 3, not able to promote wound healing in mammalian host cells. |
| Protein Name | Histatin-3Basic Histidine-Rich ProteinHstHistatin 3Hst 3Histidine-Rich Protein 3Pb Cleaved Into - Histatin-3 - His3-(20-44-PeptideHis3 20/44His3-(1-25-PeptideHis3 1/25Histatin 6Histatin-3 1/25Histatin-6 - His3-(20-43-PeptideHis3 20/43His3-(1-24-PeptideHis3 1/24Histatin 5Hst 5Histatin-3 1/24Histatin-5 - His3-(20-32-PeptideHis3 20/32His3-(1-13-PeptideHis3 1/13Histatin-3 1/13 - His3-(20-31-PeptideHis3 20/31His3-(1-12-PeptideHis3 1/12Histatin-3 1/12 - His3-(20-30-PeptideHis3 20/30His3-(1-11-PeptideHis3 1/11Histatin-3 1/11 - His3-(24-32-PeptideHis3 24/32His3-(5-13-PeptideHis3 5/13Histatin-3 5/13 - His3-(24-31-PeptideHis3 24/31His3-(5-12-PeptideHis3 5/12Histatin 11Histatin-11Histatin-3 5/12 - His3-(24-30-PeptideHis3 24/30His3-(5-11-PeptideHis3 5/11Histatin 12Histatin-12Histatin-3 5/11 - His3-(25-32-PeptideHis3 25/32His3-(6-13-PeptideHis3 6/13Histatin-3 6/13 - His3-(25-30-PeptideHis3 25/30His3-(6-11-PeptideHis3 6/11Histatin-3 6/11 - His3-(26-32-PeptideHis3 26/32His3-(7-13-PeptideHis3 7/13Histatin-3 7/13 - His3-(26-31-PeptideHis3 26/31His3-(7-12-PeptideHis3 7/12Histatin-3 7/12 - His3-(26-30-PeptideHis3 26/30His3-(7-11-PeptideHis3 7/11Histatin-3 7/11 - His3-(31-51-PeptideHis3 31/51His3-(12-32-PeptideHis3 12/32Histatin 4Histatin-3 12/32Histatin-4 - His3-(31-44-PeptideHis3 31/44His3-(12-25-PeptideHis3 12/25Histatin 9Histatin-3 12/25Histatin-9 - His3-(31-43-PeptideHis3 31/43His3-(12-24-PeptideHis3 12/24Histatin 7Histatin-3 12/24Histatin-7 - His3-(32-44-PeptideHis3 32/44His3-(13-25-PeptideHis3 13/25Histatin 10Histatin-10Histatin-3 13/25 - His3-(32-43-PeptideHis3 32-43His3-(13-24-PeptideHis3 13/24Histatin 8Histatin-3 13/24Histatin-8 - His3-(33-44-PeptideHis3 33/44His3-(14-25-PeptideHis3 14/25Histatin-3 14/25 - His3-(33-43-PeptideHis3 33/43His3-(14-24-PeptideHis3 14/24Histatin-3 14/24 - His3-(34-44-PeptideHis3 34/44His3-(15-25-PeptideHis3 15/25Histatin-3 15/25 - His3-(34-43-PeptideHis3 34/43His3-(15-24-PeptideHis3 15/24Histatin-3 15/24 - His3-(45-51-PeptideHis3 45/51His3-(26-32-PeptideHis3 26/32Histatin-3 26/32 - His3-(47-51-PeptideHis3 47/51His3-(28-32-PeptideHis3 28/32Histatin-3 28/32 - His3-(48-51-PeptideHis3 48/51His3-(29-32-PeptideHis3 29/32Histatin-3 29/32 |
| Database Links | Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedSecreted By Serous Acinar And Demilune CellsHis3-(20-43)-Peptide: SecretedMitochondrionTargeted To Mitochondria In Pathogen Cells After InternalizationAlso Localized In The Vacuole In Pathogen CellsAlso Co-Localized With Mitochondria After Internalization In Host Mammalian Cells |
| Alternative ELISA Names | Histatin-3 ELISA kitBasic Histidine-Rich Protein ELISA kitHst ELISA kitHistatin 3 ELISA kitHst 3 ELISA kitHistidine-Rich Protein 3 ELISA kitPb Cleaved Into - Histatin-3 - His3-(20-44-Peptide ELISA kitHis3 20/44 ELISA kitHis3-(1-25-Peptide ELISA kitHis3 1/25 ELISA kitHistatin 6 ELISA kitHistatin-3 1/25 ELISA kitHistatin-6 - His3-(20-43-Peptide ELISA kitHis3 20/43 ELISA kitHis3-(1-24-Peptide ELISA kitHis3 1/24 ELISA kitHistatin 5 ELISA kitHst 5 ELISA kitHistatin-3 1/24 ELISA kitHistatin-5 - His3-(20-32-Peptide ELISA kitHis3 20/32 ELISA kitHis3-(1-13-Peptide ELISA kitHis3 1/13 ELISA kitHistatin-3 1/13 - His3-(20-31-Peptide ELISA kitHis3 20/31 ELISA kitHis3-(1-12-Peptide ELISA kitHis3 1/12 ELISA kitHistatin-3 1/12 - His3-(20-30-Peptide ELISA kitHis3 20/30 ELISA kitHis3-(1-11-Peptide ELISA kitHis3 1/11 ELISA kitHistatin-3 1/11 - His3-(24-32-Peptide ELISA kitHis3 24/32 ELISA kitHis3-(5-13-Peptide ELISA kitHis3 5/13 ELISA kitHistatin-3 5/13 - His3-(24-31-Peptide ELISA kitHis3 24/31 ELISA kitHis3-(5-12-Peptide ELISA kitHis3 5/12 ELISA kitHistatin 11 ELISA kitHistatin-11 ELISA kitHistatin-3 5/12 - His3-(24-30-Peptide ELISA kitHis3 24/30 ELISA kitHis3-(5-11-Peptide ELISA kitHis3 5/11 ELISA kitHistatin 12 ELISA kitHistatin-12 ELISA kitHistatin-3 5/11 - His3-(25-32-Peptide ELISA kitHis3 25/32 ELISA kitHis3-(6-13-Peptide ELISA kitHis3 6/13 ELISA kitHistatin-3 6/13 - His3-(25-30-Peptide ELISA kitHis3 25/30 ELISA kitHis3-(6-11-Peptide ELISA kitHis3 6/11 ELISA kitHistatin-3 6/11 - His3-(26-32-Peptide ELISA kitHis3 26/32 ELISA kitHis3-(7-13-Peptide ELISA kitHis3 7/13 ELISA kitHistatin-3 7/13 - His3-(26-31-Peptide ELISA kitHis3 26/31 ELISA kitHis3-(7-12-Peptide ELISA kitHis3 7/12 ELISA kitHistatin-3 7/12 - His3-(26-30-Peptide ELISA kitHis3 26/30 ELISA kitHis3-(7-11-Peptide ELISA kitHis3 7/11 ELISA kitHistatin-3 7/11 - His3-(31-51-Peptide ELISA kitHis3 31/51 ELISA kitHis3-(12-32-Peptide ELISA kitHis3 12/32 ELISA kitHistatin 4 ELISA kitHistatin-3 12/32 ELISA kitHistatin-4 - His3-(31-44-Peptide ELISA kitHis3 31/44 ELISA kitHis3-(12-25-Peptide ELISA kitHis3 12/25 ELISA kitHistatin 9 ELISA kitHistatin-3 12/25 ELISA kitHistatin-9 - His3-(31-43-Peptide ELISA kitHis3 31/43 ELISA kitHis3-(12-24-Peptide ELISA kitHis3 12/24 ELISA kitHistatin 7 ELISA kitHistatin-3 12/24 ELISA kitHistatin-7 - His3-(32-44-Peptide ELISA kitHis3 32/44 ELISA kitHis3-(13-25-Peptide ELISA kitHis3 13/25 ELISA kitHistatin 10 ELISA kitHistatin-10 ELISA kitHistatin-3 13/25 - His3-(32-43-Peptide ELISA kitHis3 32-43 ELISA kitHis3-(13-24-Peptide ELISA kitHis3 13/24 ELISA kitHistatin 8 ELISA kitHistatin-3 13/24 ELISA kitHistatin-8 - His3-(33-44-Peptide ELISA kitHis3 33/44 ELISA kitHis3-(14-25-Peptide ELISA kitHis3 14/25 ELISA kitHistatin-3 14/25 - His3-(33-43-Peptide ELISA kitHis3 33/43 ELISA kitHis3-(14-24-Peptide ELISA kitHis3 14/24 ELISA kitHistatin-3 14/24 - His3-(34-44-Peptide ELISA kitHis3 34/44 ELISA kitHis3-(15-25-Peptide ELISA kitHis3 15/25 ELISA kitHistatin-3 15/25 - His3-(34-43-Peptide ELISA kitHis3 34/43 ELISA kitHis3-(15-24-Peptide ELISA kitHis3 15/24 ELISA kitHistatin-3 15/24 - His3-(45-51-Peptide ELISA kitHis3 45/51 ELISA kitHis3-(26-32-Peptide ELISA kitHis3 26/32 ELISA kitHistatin-3 26/32 - His3-(47-51-Peptide ELISA kitHis3 47/51 ELISA kitHis3-(28-32-Peptide ELISA kitHis3 28/32 ELISA kitHistatin-3 28/32 - His3-(48-51-Peptide ELISA kitHis3 48/51 ELISA kitHis3-(29-32-Peptide ELISA kitHis3 29/32 ELISA kitHistatin-3 29/32 ELISA kitHTN3 ELISA kitHIS2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
