| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47ng/mL |
| Detection Limit: |
0.78~50ng/mL |
| Short Description: |
This HSPB1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
HSPB1 |
| Gene ID: |
3315 |
| Uniprot ID: |
HSPB1_HUMAN |
| Specificity: |
This kit recognizes Human HSP-27/HSPB1 in samples. No significant cross-reactivity or interference between Human HSP-27/HSPB1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| Function | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
| Protein Name | Heat Shock Protein Beta-1Hspb128 Kda Heat Shock ProteinEstrogen-Regulated 24 Kda ProteinHeat Shock 27 Kda ProteinHsp 27Stress-Responsive Protein 27Srp27 |
| Database Links | Reactome: R-HSA-4420097Reactome: R-HSA-450408Reactome: R-HSA-5687128Reactome: R-HSA-9009391 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonSpindleCytoplasmic In Interphase CellsColocalizes With Mitotic Spindles In Mitotic CellsTranslocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles |
| Alternative ELISA Names | Heat Shock Protein Beta-1 ELISA kitHspb1 ELISA kit28 Kda Heat Shock Protein ELISA kitEstrogen-Regulated 24 Kda Protein ELISA kitHeat Shock 27 Kda Protein ELISA kitHsp 27 ELISA kitStress-Responsive Protein 27 ELISA kitSrp27 ELISA kitHSPB1 ELISA kitHSP27 ELISA kitHSP28 ELISA kit |
| Specificity | This kit recognizes Human HSP-27/HSPB1 in samples. No significant cross-reactivity or interference between Human HSP-27/HSPB1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 102 |
| EDTA plasma (n=8) | 87-98 | 92 |
| Cell culture media (n=8) | 83-95 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.50 | 6.90 | 21.00 | 2.60 | 7.20 | 22.30 |
| Standard deviation | 0.20 | 0.30 | 1.00 | 0.20 | 0.30 | 1.00 |
| CV (%) | 8.00 | 4.35 | 4.76 | 7.69 | 4.17 | 4.48 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
