| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.55ng/mL |
| Detection Limit: | 1.56-100ng/mL |
| Short Description : | This HSP90aA1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | HSP90AA1 |
| Gene ID: | 3320 |
| Uniprot ID: | HS90A_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | ISGylated. S-nitrosylated.negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. Ubiquitinated via 'Lys-63'-linked polyubiquitination by HECTD1. Ubiquitination promotes translocation into the cytoplasm away from the membrane and secretory pathways. |
| Function | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochondrial outer membrane which promotes host antiviral response. (Microbial infection) Seems to interfere with N.meningitidis NadA-mediated invasion of human cells. Decreasing HSP90 levels increases adhesion and entry of E.coli expressing NadA into human Chang cells.increasing its levels leads to decreased adhesion and invasion. |
| Protein Name | Heat Shock Protein Hsp 90-AlphaHeat Shock 86 KdaHsp 86Hsp86Heat Shock Protein Family C Member 1Lipopolysaccharide-Associated Protein 2Lap-2Lps-Associated Protein 2Renal Carcinoma Antigen Ny-Ren-38 |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1474151Reactome: R-HSA-168928Reactome: R-HSA-192905Reactome: R-HSA-2029482Reactome: R-HSA-203615Reactome: R-HSA-2565942Reactome: R-HSA-3000484Reactome: R-HSA-3371497Reactome: R-HSA-3371511Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-399954Reactome: R-HSA-4420097Reactome: R-HSA-5218920Reactome: R-HSA-5336415Reactome: R-HSA-5601884Reactome: R-HSA-5620912Reactome: R-HSA-5637810Reactome: R-HSA-5675482Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-8852276Reactome: R-HSA-8854518Reactome: R-HSA-8863795Reactome: R-HSA-8939211Reactome: R-HSA-9009391Reactome: R-HSA-9013418Reactome: R-HSA-9018519Reactome: R-HSA-9613829Reactome: R-HSA-9634285Reactome: R-HSA-9646399Reactome: R-HSA-9652282Reactome: R-HSA-9664565Reactome: R-HSA-9665233Reactome: R-HSA-9665244Reactome: R-HSA-9665245Reactome: R-HSA-9665246Reactome: R-HSA-9665247Reactome: R-HSA-9665249Reactome: R-HSA-9665250Reactome: R-HSA-9665251Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9665737Reactome: R-HSA-9679191Reactome: R-HSA-9705671Reactome: R-HSA-9820962Reactome: R-HSA-9834752 |
| Cellular Localisation | NucleusCytoplasmMelanosomeCell MembraneMitochondrionIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative ELISA Names | Heat Shock Protein Hsp 90-Alpha ELISA kitHeat Shock 86 Kda ELISA kitHsp 86 ELISA kitHsp86 ELISA kitHeat Shock Protein Family C Member 1 ELISA kitLipopolysaccharide-Associated Protein 2 ELISA kitLap-2 ELISA kitLps-Associated Protein 2 ELISA kitRenal Carcinoma Antigen Ny-Ren-38 ELISA kitHSP90AA1 ELISA kitHSP90A ELISA kitHSPC1 ELISA kitHSPCA ELISA kit |
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Information sourced from Uniprot.org