| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human HSF2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
HSF2 |
| Gene ID: |
3298 |
| Uniprot ID: |
HSF2_HUMAN |
| Specificity: |
This kit recognizes Human HSF2 in samples. No significant cross-reactivity or interference between Human HSF2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
| Protein Name | Heat Shock Factor Protein 2Hsf 2Heat Shock Transcription Factor 2Hstf 2 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic During Normal Growth And Moves To The Nucleus Upon Activation |
| Alternative CLIA Names | Heat Shock Factor Protein 2 CLIA kitHsf 2 CLIA kitHeat Shock Transcription Factor 2 CLIA kitHstf 2 CLIA kitHSF2 CLIA kitHSTF2 CLIA kit |
| Specificity | This kit recognizes Human HSF2 in samples. No significant cross-reactivity or interference between Human HSF2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 93 |
| EDTA plasma(n=8) | 89-101 | 96 |
| Cell culture media(n=8) | 89-101 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 100.38 | 179.86 | 719.91 | 93.41 | 167.67 | 654.71 |
| Standard deviation | 12.16 | 20.90 | 48.38 | 8.90 | 13.58 | 41.31 |
| CV (%) | 12.11 | 11.62 | 6.72 | 9.53 | 8.10 | 6.31 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
