• Human HSF2 (Heat shock factor protein 2) CLIA Kit (STJC0000813)

Human HSF2 (Heat shock factor protein 2) CLIA Kit (STJC0000813)

SKU:
STJC0000813

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human HSF2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: HSF2
Gene ID: 3298
Uniprot ID: HSF2_HUMAN
Specificity: This kit recognizes Human HSF2 in samples. No significant cross-reactivity or interference between Human HSF2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Protein Name Heat Shock Factor Protein 2
Hsf 2
Heat Shock Transcription Factor 2
Hstf 2
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic During Normal Growth And Moves To The Nucleus Upon Activation
Alternative CLIA Names Heat Shock Factor Protein 2 CLIA kit
Hsf 2 CLIA kit
Heat Shock Transcription Factor 2 CLIA kit
Hstf 2 CLIA kit
HSF2 CLIA kit
HSTF2 CLIA kit
Specificity This kit recognizes Human HSF2 in samples. No significant cross-reactivity or interference between Human HSF2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 93
EDTA plasma(n=8) 89-101 96
Cell culture media(n=8) 89-101 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.38 179.86 719.91 93.41 167.67 654.71
Standard deviation 12.16 20.90 48.38 8.90 13.58 41.31
CV (%) 12.11 11.62 6.72 9.53 8.10 6.31
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance